Abstract: BACKGROUND : Cephalosporins and ciprofloxacin are commonly used in the treatment of gram negative bacilli infections. Third generation cephalosporins are first choice but expensive than ciprofloxacin. So there is a need to compare their susceptibility. OBJECTIVE: To compare the susceptibility of gram negative bacilli clinical isolates to cephalosporins and ciprofloxacin. Susceptibility of ESBL and AmpC producers to ciprofloxacin. METHODOLOGY: Disk diffusion method performed on100 Gram negative bacilli isolates. Susceptibilityto cefuroxime, cefotaxime, ceftazidime, ceftazidime with clavulanic acid, cefoxitin, cefepime and ciprofloxacin were tested. ESBL detected by>7mm zone with ceftazidime clavulanic acid than ceftazidime alone. AmpC detected by cefoxitin resistant and cefepime sensitive. RESULTS: Pseudomonas isolates exudate7(29%),burns13(54%), bood4(17%).Susceptibility to ciprofloxacin, cefepime in exudate 4(57%),3(43%),burns 5(38%),4(31%) respectively. Blood isolates susceptible to cefepime3(75%)other cephalosporins and ciprofloxacin2(50%). Escherichia coli isolates 7(28%)exudates,3(12%)blood,7(28%)sputum and 9(36%)urine. Blood isolates100% susceptibility to ciprofloxacin, cefepime Exudate 2(28%),4(57%). Equal susceptibility to both 4(57%)sputum. Urine 6(66%),7(78%) respectively. Klebsiella 10(32%) exudates and burns,10(32%)sputum,2(6%)blood. Their susceptibility to ciprofloxacin 2(22%),1(11%),5(50%) and cefepime2(22%),2(22%),1(50%),5(50%) respectively. Enterobacter blood 6(50%), exudate, burns sputum2(17%)each. Susceptibility to ciprofloxacin2(100%) exudates and burns, 4(67%)blood. Resistance to cefepime seen in all except blood isolates4(67%). Citrobacter isolates from exudates3(60%), from blood and urine1(20%) each.Exudate1(33%) sensitive to all cephalosporins and ciprofloxacin. Blood,urine1(100%) sensitive to ciprofloxacin. Acinetobacter 3(100%) exudates.1(33%) sensitive only to cefepime Ciprofloxacin susceptibility seen in Pseudomonas54%,E.coli72%,klebsiella29%,Enterobacter66%,Citrobacter80% and acinetobacter resistant and cefepime susceptibility 21%,60%,25%,41%,40% and 33% respectively. Among 8 ESBL, 5AmpC producers 4(50%) and 2(40%) sensitive to ciprofloxacin respectively.